1）Production of protein

Expressing exogenous proteins in E. coli cells is the routine work in our lab. The details include gene design, plasmid construction and protein expression&purification. Sometimes, we used phage-display to screen scFv antibodies for research purposes.

2）Serial application of membrane penetrating peptides

Antimicrobial peptides could spontaneously intercalate into biomembrane including both prokaryotic and eukaryotic cells. AMPs could be used as antimicrobial agents, and could also be used to decorate cellular membrane for bacterial discrimination and targeting delivery. Besides, AMPs could be used to opsonize pathogens in fusion with a common vaccine against infectious diseases. These applications have been subject to patent application.

3）Nucleic acid detection

We are trying to design a new approach for quick detection of single chain nucleic acid based on strand displacement/exchange. This method could be used for DNA/RNA detection and detection of cellular in situ viral infection.

4）Screening of antimicrobial compounds

Design, synthesis and application of antibacterial and targeted membrane penetrating peptides, Science and Technology Second Prize, Jiangsu Medical Association, 2018

1)Fan X#, Xu H, Zhao F, Song J, Jin Y, Zhang C, Wu G*. Lipid-mimickingpeptide decorates erythrocyte membrane for active delivery to engraftedMDA-MB-231 breast tumour. Eur J Pharm Biopharm. 2020 May 3; doi:10.1016/j.ejpb.2020.04.024.

2)Fan X#*, Xu H, Song J, Jin Y, Wink M, Wu G*. Using aMembrane-Penetrating-Peptide to Anchor Ligands in the Liposome MembraneFacilitates Targeted Drug Delivery. Bioconjug Chem. 2020 Jan 15;31(1):113-122.

3)Fan X#*, Xu W, Gao W, Jiang X, Wu G*. A facile method to classify clinicisolates with a turn-off sensor array based on grapheme oxide and antimicrobialpeptides. Sensors and Actuators B-Chemical. 2020 307:127607.

4)Jiang X#, Fan X, Xu W, Zhao C, Wu H, Zhang R, WuG*. Self-assembled peptide nanoparticles responsive to multiple tumormicroenvironment triggers provide highly efficient targeted delivery andrelease of antitumor drug. J Control Release. 2019 Dec 28;316:196-207.

5)Fan X#, Xu W, Han J, Jiang X, Wink M, Wu G*. Antimicrobial peptidehybrid fluorescent protein based sensor array discriminate ten most frequentclinic isolates. Biochim Biophys Acta Gen Subj. 2019 Jun;1863(6):1158-1166.

6)Fan X#*, Korytowski A, Makky A, Tanaka M, Wink M*. Ib-AMP4 insertioncauses surface rearrangement in the phospholipid bilayer of biomembranes:Implications from quartz-crystal microbalance with dissipation. Biochim BiophysActa Biomembr. 2018 Feb;1860(2):617-623.

7)Xue X#, Fan X#, Qu Q, Wu G*.Bioscreening and expression of a camel anti-CTGF VHH nanobody and itsrenaturation by a novel dialysis-dilution method. AMB Express. 2016Dec;6(1):72.

8)Fan X#, Fan J#, Wang X, Wu P, Wu G*. S-thanatinfunctionalized liposome potentially targeting on Klebsiella pneumoniae and itsapplication in sepsis mouse model. Front Pharmacol. 2015;6:249.