研究方向和兴趣：

实验室的研究方向主要集中在对动物在生理、病理及行为等变化下所涉及的神经系统、内分泌系统、消化系统和生殖系统的相关机制的研究方面。研究对象包括围绕生物钟、神经内分泌轴、胰岛、肠道菌群和生殖系统为主要研究对象的相关生理（如精子生成）、病理（如男性不育和脑卒中）的机制。

实验室的兴趣主要围绕着由神经系统和外周组织器官共同协调起来的神经内分泌轴展开，包括动物行为学，认知，相关疾病的发生发展，等等。另外，在技术领域，实验室对新的高通量精确核酸定量技术和新型在体内基因学操作技术的研发等也具有相当浓厚的兴趣。实验室通过使用多种动物模型和基因学工具，结合行为学和生物化学、分子生物学、生物信息学等跨领域技术手段开展相关研究工作。实验室感兴趣的几个具体研究方向包括基础研究和技术研发两大类，若干具体方向：

1）基础研究：

l神经肽或生长因子等信号对神经系统，动物行为，和生殖系统的影响。

l寻找针对脑卒中的潜在诊疗分子及其机制研究。

l寻找改善糖尿病相关并发症的诊疗分子及其机制研究。

l宿主和菌群之间的相互关系。

2）应用研究（新技术研发）：

l在体靶向病毒

l基因编辑技术

l光/磁遗传技术

lB-细胞展示技术

l原位核酸放大技术

东南大学网页：http://jszy.seu.edu.cn/_s1454/main.psp  

Licenses / patents:

Zhao S*and Fernald RD. Real-time PCR Miner for Real-time PCR Quantification.

2004, Stanford License #S04-261, Patent pending.

http://otlportal.stanford.edu/techfinder/technology/ID=24142/http://www.ibridgenetwork.org/stanford/real-time-pcr-miner

http://www.miner.ewindup.info

Publications:

Till 2018/11, total 1649 cited (stat by ResearchGate).

https://www.researchgate.net/profile/Sheng_Zhao3

1.WangXH, Li W, Chen J, Qiu SH, Yin H, Zhao S, Carvalho V, Zhou YT, Shi RF, Hu JN, LiSY, Nijiati M, Sun ZL*. A Transcriptional Sequencing Analysis of Islet StellateCell and Pancreatic Stellate Cell, Journal of Diabetes Research, 2018.1.24,2018

2.SongH, Wang D, De Jesus Perez F, Xie R, Liu Z, Chen CC, Yu M, Yuan L, Fernald RD, Zhao S^*. (Jul 2017) Rhythmicexpressed clock regulates the transcription of proliferating cellular nuclearantigen in teleost retina. Exp Eye Res. 160: 21-30.

3.SongH, Zhang Y, Liu N, Zhang D, Wan C, ZhaoS, Kong Y, Yuan L. (Dec 2016) Let-7b inhibits the malignant behavior ofglioma cells and glioma stem-like cells via downregulation of E2F2. J PhysiolBiochem. 72(4): 733-744.

4.SongH, Zhang Y, Liu N, Zhao S, Kong Y,Yuan L. (Oct 2016) miR-92a-3p Exerts Various Effects in Glioma and GliomaStem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt SignalingPathways. Int J Mol Sci. 17(11). pii: E1799.

5.SongH, Zhang Y, Liu N, Wan C, Zhang D, ZhaoS, Kong Y, Yuan L. (Jun 2016) miR-92b regulates glioma cells proliferation,migration, invasion, and apoptosis via PTEN/Akt signaling pathway. J PhysiolBiochem. 72(2):201-211.

6.LiS, Wang Q, Qiang Q, Shan H, Shi M, Chen B, ZhaoS, Yuan L. (Nov 2015) Sp1-mediated transcriptional regulation of MALAT1plays a critical role in tumor. J Cancer Res Clin Oncol. 141(11):1909-1920.

7.GeraghtyAC, Muroy SE, Zhao S, Bentley GE,Kriegsfeld LJ, Kaufer D*. (Jan. 2015) Knockdown of hypothalamic RFRP3 preventschronic stress-induced infertility and embryo resorption. eLife 4, e04316.

8.PiekarskiDJ, Zhao S, Jennings KJ, Iwasa T,Legan SJ, Mikkelsen JD, Tsutsui K, Kriegsfeld LJ*. (Jul 2013) Gonadotropin-inhibitoryhormone reduces sexual motivation but not lordosis behavior in female Syrianhamsters (Mesocricetus auratus). Hormone Behavior. 64(3), 501-510.

9.RuijterJM*, Pfaffl MW, Zhao S, Spiess AN,Boggy G, Blom J, Rutledge RG, Sisti D, Lievens A, De Preter K, Derveaux S,Hellemans J, Vandesompele J. (Jan 2013) Evaluation of qPCR curve analysismethods for reliable biomarker discovery: Bias, resolution, precision, andimplications. Methods. 59(1), 32-46.

10.KriegsfeldLJ*,Gibson EM, Williams, WP, Zhao S, Mason AO, Bentley GE, Tsutsui, K(Jul 2010) The Roleof RFamide-Related Peptide-3 (RFRP-3) in Mammalian Reproductive Function andBehavior. Journal of Neuroendocrinology. 22(7), 692-700.

11.MasonAO, Duffy S, Zhao S, Ubuka T,Bentley GE, Tsutsui K, Silver R, Kriegsfeld LJ*(Jun 2010)Photoperiod and Reproductive Condition Are Associated with Changes inRFamide-Related Peptide (RFRP) Expression in Syrian Hamsters (Mesocricetusauratus)., Journal of Biological Rhythms, 25(3), 176-185.

12.Zhao S, Kelm RJ Jr, Fernald RD* (Mar 2010)Regulation of Gonadotropin Releasing Hormone 1 Gene Transcription by Members ofthe Purine-Rich Element Binding Protein Family., American Journal of Physiology.Endocrinology and Metabolism, 298(3), E524-533.

13.Zhao S, Zhu E, Yang C, Bentley GE, Tsutsui K, andKriegsfeld LJ* (Feb 2010) RFamide-Related Peptide and Messenger RibonucleicAcid Expression in Mammalian Testis: Association with the Spermatogenic Cycle.,Endocrinology, 151(2), 617-627.

14.Zhao S and Kriegsfeld LJ* (Jan 2009) Daily changesin GT1–7 cell sensitivity to GnRH secretagogues that trigger ovulation., Neuroendocrinology,89(4), 448-457.

15.GibsonEM, Humber SA, Jain S, Williams WP, ZhaoS, Bentley GE, Tsutsui K, Kriegsfeld LJ* (Oct 2008) Alterations in RFamide-relatedpeptide expression are coordinated with the preovulatory luteinizing hormonesurge., Endocrinology, 149(10), 4958-4969.

16.KorzanWJ, Robison RR, Zhao S, Fernald RD*(Sep 2008) Color change as a potential behavioral strategy., Hormones and behavior,54(3), 463-470.

17.Zhao S, Korzan WJ, Chen CC, Fernald RD* (Jan 2008)Heterogeneous nuclear ribonucleoprotein A/B and G inhibits the transcription ofgonadotropin releasing hormone 1., Molecular and cellular neuroscience, 37(1),69-84.

18.GroneBP, Zhao S, Chen CC, Fernald RD*(Dec 2007) Localization and diurnal expression of melanopsin, vertebrateancient opsin, and pituitary adenylate cyclase-activating peptide mRNA in ateleost retina., Journal of biological rhythms, 22(6), 558-561.

19.BrillJ, Lee M, Zhao S, Fernald RD, andHuguenard JR*. (Jun 2006) Chronic valproic acid treatment triggers increasedneuropeptide y expression and signaling in rat nucleus reticularis thalami. Journalof Neuroscience. 26(25), 6813-6822.

20.Zhao S^* and Fernald RD. (Oct 2005) Comprehensivealgorithm for quantitative real-time polymerase chain reaction. Journal of ComputationalBiology. 12(8), 1045-1062.

21.Zhao S, Yang YN, Song JG*. (Apr 2004) Ceramideinduces caspase-dependent and -independent apoptosis in A-431 cells. Journal ofCellular Physiology. 199(1), 47-56.

22.YangY, Zhao S, Song J*. (Feb 2004)Caspase-dependent apoptosis and -independent poly (ADP-ribose) polymerasecleavage induced by transforming growth factor beta1. International Journal ofBiochemistry & Cell Biology. 36(2), 223-234.

23.ChenHH, Zhao S, Song JG*. (May 2003)TGF-beta1 suppresses apoptosis via differential regulation of MAP kinases andceramide production. Cell Death and Differentiation. 10(5), 516-527.

24.Zhao S, Du XY, Chen JS, Zhou YC, Song JG*. (Oct2002) Secretory phospholipase A(2) inhibits epidermal growth factor-inducedreceptor activation. Experimental Cell Research. 279(2), 354-364.

25.Zhao S, Du XY, Chai MQ, Chen JS, Zhou YC, Song JG*.(Apr 2002) Secretory phospholipase A(2) induces apoptosis via a mechanisminvolving ceramide generation. Biochimica Et Biophysica Acta-Molecular and CellBiology of Lipids. 1581(3), 75-88.

26.ChaiMQ, Chen JS, Zhao S, Song JG*. (Sep2001) Propranolol increases phosphatidic acid level via activation ofphospholipase D. Acta Pharmacologica Sinica. 22(9), 777-784.

27.LiaoJH, Chen JS, Chai MQ, Zhao S, SongJG*. (Jun 2001) The involvement of p38 MAPK in transforming growth factorbeta1-induced apoptosis in murine hepatocytes. Cell Research. 11(2),89-94.

28.ChenJS, Chai MQ, Chen HH, Zhao S, SongJG*. (Nov 2000) Regulation of phospholipase D activity and ceramide productionin daunorubicin-induced apoptosis in A-431 cells. Biochimica Et Biophysica Acta- Molecular and Cell Biology of Lipids. 1488(3), 219-232.

29.ZhouBH, Chen JS, Chai MQ, Zhao S, LiangJ, Chen HH, Song JG*. (Jun 2000) Activation of phospholipase D activity intransforming growth factor-beta-induced cell growth inhibition. Cell Research.10(2), 139-149.