胡娟                  
博士生导师 化学化工学院                  
联系电话： 办公时间：                                          
传 真：                                           主页网址：                                          
办公地点：九龙湖校区化工新楼317                                           电子邮箱：hujuan@seu.edu.cn                                          
通讯地址：南京市江宁区东南大学路2号化学化工学院                                          
研究方向
荣誉奖励




课题组招收硕、博士研究生，欢迎报考！

联系方式邮箱：hujuan@seu.edu.cn

个人简介

胡娟，博士，化学化工学院硕、博士生导师。主要研究领域为生化分析和单分子检测研究。主持完成国家自然科学基金面上项目、青年科学基金项目，参与国家自然科学

基金重点项目和重大科研仪器研制项目等。已在ACS Nano、Nano Letters、Chemical Science、Analytical Chemistry、Biosensors and Bioelectronics等国际权威期刊上发表

论文50余篇，其中JCR一区38篇，自然指数(Nature Index)期刊论文为26篇，累计影响因子387.4，论文总被引用次数超过1900次，H指数为25。获得山东省自然科学奖二等奖、

深圳市自然科学奖二等奖等，获国家授权发明专利10余项。

科研项目

1.  国家自然科学基金-面上项目，表面增强拉曼光谱及成像技术在肿瘤细胞检测中的应用研究，2016-01至2019-12，主持

2.  国家自然科学基金-青年基金，基于表面增强拉曼光谱检测生物标志物技术的研究，2013-01至2015-12，主持

3.  国家自然科学基金-重点项目，靶向肺癌循环肿瘤细胞的单分子检测技术研究，2018-01至2022-12，参加

4.  山东省高等学校科技计划项目，肿瘤生物标志物拉曼光谱传感方法的研究与应用，2017-01至2018-12，主持

论文

1.  Meng Liu, Zi-wei Han, Su Jiang, Yun Han, Hao Liu, Di Zhang, Juan Hu,*  Qinfeng Xu,* Chun-yang Zhang,* Construction of a transcription-driven CRISPR  RNA auto-generation-mediated CRISPR-Cas12a system for sensitive detection of  endogenous repair glycosylase, Sensors  and Actuators B: Chemical, 2024, 405, 135401. (JCR一区,  IF: 8.4)

2.  Qian Zhang, Juan Hu, Dong-ling Li, Jian-Ge Qiu, Bing-Hua Jiang,*  Chun-yang Zhang,* Construction of single-molecule counting-based biosensors  for DNA-modifying enzymes: A review, Analytica  Chimica Acta, 2024, 342395. (JCR一区,  IF: 6.2)

3.  Maoding Zuo, Yuncong Yang, Su Jiang, Chenyu Zhu, Yun Han, Juan Hu,  Kewei Ren,* Lin Cui,* Chun-Yang Zhang,* Ultrathin-FeOOH-coated MnO2 nanozyme  with enhanced catalase-like and oxidase-like activities for  photoelectrochemical and colorimetric detection of organophosphorus  pesticides, Food Chemistry, 2024,  445, 138716. (JCR一区, IF: 8.8)

4.  Jin-zhi Zhang, Ning-ning Zhao, Zi-yue Wang, Juan Hu,* Chun-yang Zhang,*  Target-activated T7 transcription circuit-mediated multiple cycling signal  amplification for monitoring of flap endonuclease 1 activity in cancer cells.  Analyst, 2023, 148(12), 2732-2738.  (JCR二区, IF: 4.2)

5.  Ming-hao Liu, Feng-zheng Li, Wen-jing Liu, Juan Hu, Meng Liu, Chun-Yang  Zhang,* An electrostatic force-independent dephosphorylation-driven  chemiluminescent biosensor for sensitive and rapid detection of  poly(ADP-ribose) polymerase-1 in human breast tissues. Chemical Engineering Journal, 2023, 460, 141776. (JCR一区,  IF: 15.1)

6.  Shuangshuang Zhang, Jin-an Hou, Juan Hu,* Chun-Yang Zhang,* In-situ self-catalyzed  growth of manganese embedded 3D flakes-coated carbon rod as an efficient oxygen  reduction reaction catalyst of zinc-air batteries. Chemistry-A European Journal, 2023, 29(5), e202202989. (JCR二区,  IF: 4.3)

7.  Xiao-wen Liu, Wen-jing Liu, Yingying Meng, Juan Hu,* Chun-yang Zhang,*  Development of a tandem signal amplification strategy for label-free sensing  polynucleotide kinase activity in cancer cells. Talanta, 2023, 253, 124001. (JCR一区,  IF: 6.1)

8.  Lin Cui, Chen-yu Zhu, Juan Hu, Xiao-meng Meng, Mengmeng Jiang, Wenqiang  Gao,* Xiaolei Wang,* Chun-yang Zhang,* Construction of a dual-mode biosensor  for electrochemiluminescent and electrochemical sensing of alkaline  phosphatase. Sensors and Actuators  B-Chemical, 2023, 374, 132779. (JCR一区,  IF: 8.4)

9.  Su Jiang, Qian Liu, Juan Hu, Dong Yuan, Yan Zhang,* Chun-yang Zhang,*  Target-triggered assembly of functional G-quadruplex DNAzyme nanowires for  sensitive detection of miRNA in lung tissues. Sensors and Actuators B-Chemical, 2022, 373, 132689. (JCR一区,  IF: 8.4)

10.  Zi-yue Wang, Huimin Yuan, Dong-ling Li, Juan Hu,* Jian-Ge Qiu,*  Chun-yang Zhang,* Hydroxymethylation-specific ligation-mediated single  quantum dot-based nanosensors for sensitive detection of 5-hydroxymethylcytosine  in cancer cells. Analytical Chemistry,  2022, 94(27), 9785-9792. (JCR一区, IF:  7.4)

12.  Shu-hua Wei, Meng Liu, Juan Hu,* Chun-yang Zhang,* Target-initiated cascade  signal amplification lights up a G-quadruplex for a label-free detection of circular  ribonucleic acids. Analytical Chemistry,  2022, 94(25), 9193-9200. (JCR一区, IF:  7.4)

13.  Xue-ru Wei, Yingying Meng, Qinfeng Xu, Juan Hu,* Chun-yang Zhang,*  Label-free and homogeneous detection of flap endonuclease 1 by  ligation-promoted hyperbranched rolling circle amplification platform. Talanta, 2022, 243, 123342. (JCR一区, IF:  6.1)

14.  Yan Zhang, Chen Wang, Xiaoran Zou, Xiaorui Tian, Juan Hu,* Chun-yang  Zhang,* Simultaneous enzyme-free detection of multiple long noncoding RNAs in  cancer cells at single-molecule/particle level. Nano Letters, 2021, 21(10), 4193-4201. (JCR一区, IF:  10.8)

15.  Chen-chen Li, Hui-yan Chen, Xiliang Luo,* Juan Hu,* Chun-yang Zhang,*  Multicolor fluorescence encoding of different microRNAs in lung cancer  tissues at the single-molecule level. Chemical  Science, 2021, 12(37), 12407-12418. (JCR一区, IF:  8.4)

16.  Chen-chen Li, Jinping Hu, Xiliang Luo,* Juan Hu,* Chun-yang Zhang,*  Development of a single quantum dot-mediated FRET nanosensor for sensitive  detection of single-nucleotide polymorphism in cancer cells. Analytical Chemistry, 2021, 93(43),  14568-14576. (JCR一区, IF: 7.4)

17.  Chen-chen Li, Yue-hong Dong, Xiaoran Zou, Xiliang Luo,* Dazhong Shen,*  Juan Hu,* Chun-yang Zhang,* Label-free and template-free chemiluminescent  biosensor for sensitive detection of 5-hydroxymethylcytosine in genomic DNA. Analytical Chemistry, 2021, 93(4),  1939-1943. (JCR一区, IF: 7.4)

18.  Juan Hu, Wen Liu, Jufeng Wang, Jian-Ge Qiu,* Chun-yang Zhang,* Simple  mix-and-read assay with multiple cyclic enzymatic repairing amplification for  rapid and sensitive detection of DNA glycosylase. Analytical Chemistry, 2021, 93(18), 6913-6918. (JCR一区,  IF: 7.4)

19.  Juan Hu, Jie Yao, Jufeng Wang, Li-yuan Pan, Jian-Ge Qiu,* Chun-yang  Zhang,* A single quantum dot-based fluorescence resonance energy transfer  biosensor for antibody-free detection of ten-eleven translocation 1. Chemical Communications, 2021, 57(29),  3543-3546. (JCR二区, IF: 4.9)

20.  Juan Hu, Li-yuan Pan, Yueying Li, Xiaoran Zou, Bing-jie Liu, BingHua  Jiang,* Chun-yang Zhang,* Deacetylation-activated construction of single  quantum dot-based nanosensor for sirtuin 1 assay. Talanta, 2021, 224, 121918. (JCR一区,  IF: 6.1)

21.  Junjie Zhang, Xianwei Su, Ao Qi, Lulu Liu, Lijian Zhang, Yu Zhong,  Simin Xu, Ximei Liu, Juan Hu,* Yusen Chen,* Chun-yang Zhang,* Chun Cai,*  Metabolomic profiling of fatty acid biomarkers for intracerebral hemorrhage  stroke. Talanta, 2021, 222, 121679.  (JCR一区, IF: 6.1)

22.  Chen-chen Li, Hui-yan Chen, Yue-hong Dong, Xiliang Luo,* Juan Hu,*  Chun-yang Zhang,* Advances in detection of epigenetic  modification-5-hydroxymethylcytosine. Acta  Chimica Sinica, 2021, 79(5), 614-627. (JCR三区,  IF: 2.5)

23.  Chen-chen Li, Hui-yan Chen, Juan Hu,* Chun-yang Zhang,* Rolling circle  amplification-driven encoding of different fluorescent molecules for  simultaneous detection of multiple DNA repair enzymes at the single-molecule  level. Chemical Science, 2020,  11(22), 5724-5734. (JCR一区,  IF: 8.4)

24.  Yueying Li, Wen Liu, Qinfeng Xu, Juan Hu,* Chun-yang Zhang,*  Construction of a sensitive protease sensor with DNA-peptide conjugates for  single-molecule detection of multiple matrix metalloproteinases. Biosensors and Bioelectronics, 2020,  169, 112647. (JCR一区, IF: 12.6)

25.  Juan Hu, Yang Liu, Chun-yang Zhang,* Construction of a single quantum  dot nanosensor with the capability of sensing methylcytosine sites for  sensitive quantification of methyltransferase. Nanoscale, 2020, 12(7), 4519-4526. (JCR一区, IF:  6.7)

26.  Juan Hu, Wen-can Li, Jian-Ge Qiu, BingHua Jiang,* Chun-yang Zhang,* A  multifunctional DNA nanostructure based on multicolor FRET for nuclease  activity assay. Analyst, 2020, 145(18),  6054-6060. (JCR二区, IF: 4.2)

27.  Juan Hu, Ming-hao Liu, Chun-yang Zhang,* Construction of tetrahedral  DNA-quantum dot nanostructure with the integration of multistep Förster  resonance energy transfer for multiplex enzymes assay. ACS Nano, 2019, 13(6), 7191-7201. (JCR一区, IF:  17.1)

28.  Chen-chen Li, Wan-xin Liu, Juan Hu,* Chun-yang Zhang,* A single quantum  dot-based nanosensor with multilayer of multiple acceptors for ultrasensitive  detection of human alkyladenine DNA glycosylase. Chemical Science, 2019, 10(37), 8675-8684. (JCR一区,  IF: 8.4)

29.  Lin Cui, Juan Hu, Meng Wang, Chen-chen Li, Chun-yang Zhang,* Label-free  and immobilization-free electrochemical magnetobiosensor for sensitive  detection of 5-hydroxymethylcytosine in genomic DNA. Analytical Chemistry, 2019, 91(2), 1232-1236. (JCR一区,  IF: 7.4)

30.  Juan Hu, Ming-hao Liu, Chun-yang Zhang,* Integration of isothermal  amplification with quantum dot-based fluorescence resonance energy transfer  for simultaneous detection of multiple microRNAs. Chemical Science, 2018, 9(18), 4258-4267. (JCR一区,  IF: 8.4)

31.  Juan Hu, Ming-hao Liu, Ying Li, Bo Tang,* Chun-yang Zhang,*  Simultaneous sensitive detection of multiple DNA glycosylases from lung  cancer cells at the single-molecule level. Chemical Science, 2018, 9(3), 712-720. (JCR一区,  IF: 8.4)

32.  Chen-chen Li, Juan Hu, Mengfei Lu, Chun-yang Zhang,* Quantum dot-based  electrochemical biosensor for stripping voltammetric detection of telomerase  at the single-cell level. Biosensors  and Bioelectronics, 2018, 122, 51-57. (JCR一区,  IF: 12.6)

33.  Lin Cui, Juan Hu, Chen-chen Li, Cheng-ming Wang, Chun-yang Zhang,* An  electrochemical biosensor based on the enhanced quasi-reversible redox signal  of prussian blue generated by self-sacrificial label of iron metal-organic  framework. Biosensors and  Bioelectronics, 2018, 122, 168-174. (JCR一区,  IF: 12.6)

34.  Lin Cui, Juan Hu, Meng Wang, Xing-kang Diao, Chen-chen Li, Chun-yang  Zhang,* Mimic peroxidase- and Bi2S3 nanorod-based  photoelectrochemical biosensor for signal-on detection of polynucleotide  kinase. Analytical Chemistry, 2018,  90(19), 11478-11485. (JCR一区,  IF: 7.4)

35.  Dandan Zhang, Juan Hu, Xiao-yun Yang, Yanxia Wu, Wenmei Su, Chun-yang  Zhang,* Target-initiated synthesis of fluorescent copper nanoparticles for  the sensitive and label-free detection of bleomycin. Nanoscale, 2018, 10(23), 11134-11142. (JCR一区,  IF: 6.7)

36.  Juan Hu, Yueying Li, Ying Li, Bo Tang,* Chun-yang Zhang,* Single  quantum dot-based nanosensor for sensitive detection of O-GIcNAc transferase  activity. Analytical Chemistry,  2017, 89(23), 12992-12999. (JCR一区,  IF: 7.4)

37.  Juan Hu, Zi-yue Wang, Chen-chen Li, Chun-yang Zhang,* Advances in  single quantum dot-based nanosensors. Chemical  Communications, 2017, 53(100), 13284-13295. (JCR二区,  IF: 4.9)

38.  Juan Hu, Zi-Yue Wang, Chun-Yang Zhang,* Advance in quantitative  single-molecule detection. Scientia  Sinica Chimica, 2017, 47(10), 1161-1169.

39.  Si-qiang Huang, Juan Hu, Guichi Zhu, Chun-yang Zhang,* Sensitive  detection of point mutation using exponential strand displacement  amplification-based surface enhanced Raman spectroscopy. Biosensors and Bioelectronics, 2015, 65, 191-197. (JCR一区,  IF: 12.6)

40.  Li-Ping Ye, Juan Hu, Li Liang, Chun-yang Zhang,* Surface-enhanced Raman  spectroscopy for simultaneous sensitive detection of multiple microRNAs in  lung cancer cells. Chemical  Communications, 2014, 50(80), 11883-11886. (JCR二区,  IF: 4.9)

41.  Juan Hu, Chun-yang Zhang,* Multiplex detection of lung cancer cells at  the single-molecule level. Chemical  Communications, 2014, 50(88), 13581-13584. (JCR二区,  IF: 4.9)

42.  Juan Hu, Chun-yang Zhang,* Simple and accurate quantification of  quantum yield at the single-molecule/particle level. Analytical Chemistry, 2013, 85(4), 2000-2004. (JCR一区,  IF: 7.4)

43.  Ya-ping Zeng, Juan Hu, Yi Long, Chun-yang Zhang,* Sensitive detection  of DNA methyltransferase using hairpin probe-based primer generation rolling  circle amplification-induced chemiluminescence. Analytical Chemistry, 2013, 85(12), 6143-6150. (JCR一区,  IF: 7.4)

44.  Yan Zhang, Juan Hu, Chun-yang Zhang,* Sensitive detection of  transcription factors by isothermal exponential amplification-based  colorimetric assay. Analytical  Chemistry, 2012, 84(21), 9544-9549. (JCR一区,  IF: 7.4)

45.  Juan Hu, Chun-yang Zhang,* Single base extension reaction-based surface  enhanced Raman spectroscopy for DNA methylation assay, Biosensors and Bioelectronics, 2012, 31(1), 451-457. (JCR一区,  IF: 12.6)

46.  Cai-jun Cao, De-rong Li, Chao-xiong Chen, Xiao-yun Yang, Juan Hu, Yong  Yang, Chun-yang Zhang,* Multimodal optical microscopy in combination with  gold nanorods for cancer cell imaging. Journal  of Biomedical Optics, 2012, 17(12), 126002. (JCR二区,  IF: 3.5)

47.  Chun-yang Zhang,* Juan Hu, Single quantum dot-based nanosensor for  multiple DNA detection. Analytical  Chemistry, 2010, 82(5), 1921-1927. (JCR一区,  IF: 7.4)

48.  Juan Hu, Chun-yang Zhang,* Sensitive detection of nucleic acids with  rolling circle amplification and surface-enhanced Raman scattering  spectroscopy, Analytical Chemistry,  2010, 82(21), 8991-8997. (JCR一区,  IF: 7.4)

49.  Juan Hu, Chun-yang Zhang,* Surface-enhanced Raman scattering technology  and its application to gene analysis. Progress  in Chemistry, 2010, 22(8), 1641-1647. (JCR四区,  IF: 1.3)

50.  Juan Hu, Peng-Cheng Zheng, Jian-Hui Jiang, Guo-Li Shen, Ru-Qin Yu,*  Guo-Kun Liu,* Sub-attomolar HIV-1 DNA detection using surface-enhanced Raman  spectroscopy. Analyst, 2010,  135(5), 1084-1089. (JCR二区,  IF: 4.2)

51.  Juan Hu, Peng-Cheng Zheng, Jian-Hui Jiang, Guo-Li Shen, Ru-Qin Yu,*  Guo-Kun Liu,* Electrostatic interaction based approach to thrombin detection  by surface-enhanced Raman spectroscopy. Analytical  Chemistry, 2009, 81(1), 87-93. (JCR一区,  IF: 7.4)

52.  Pengcheng Zheng, Juan Hu, Guoli Shen, Jianhui Jiang, Ruqin Yu,* Guokun  Liu,* Synthesis, structure and growth mechanism of size and shape tunable  Au/Ag bimetallic nanoparticles. Chinese  Journal of Chemistry, 2009, 27(11), 2137-2144. (JCR二区, IF:  5.4)

53.  Guo-Kun Liu, Juan Hu, Peng-Cheng Zheng, Guo-Li Shen, Jian-Hui Jiang,  Ru-Qin Yu,* Yan Cui, Bin Ren,* Laser-induced formation of  metal-molecule-metal junctions between Au nanoparticles as probed by  surface-enhanced Raman spectroscopy. Journal  of Physical Chemistry C, 2008, 112(16), 6499-6508. (JCR二区,  IF: 3.7)

专利

1.  一种无标记荧光检测FEN1活性的方法，专利号ZL202210142803.5

2.  一种检测基质金属蛋白酶的生物传感器及应用，专利号ZL202010848753.3

3.  一种同时检测多种DNA糖基化酶的荧光化学传感器、其检测方法及应用，专利号ZL202010067224.X

4.  一种检测甲基转移酶的单个量子点纳米传感器及其检测方法和应用，专利号ZL201911397409.0

5.  一种检测去乙酰化酶用纳米传感器及其检测方法和应用，专利号ZL202011209095.X

6.  一种检测DNA羟化酶TET1的纳米传感器及其检测方法和应用，专利号ZL202110261946.3

7.  一种检测5-羟甲基胞嘧啶的化学发光生物传感器及其检测方法和应用，专利号ZL202011195224.4

8.  一种同时检测多种长链非编码RNA的复合物及方法，专利号ZL202110069247.9

9.  一步法荧光检测体系及DNA糖基化酶活性的检测方法和应用，专利号ZL202110361586.4

10.  基于单量子点的纳米传感器检测人烷基糖基化酶的方法，专利号ZL201910199129.2

11.  恒温扩增与量子点荧光共振能量转移联用用于同时检测多种MicroRNAs的方法，专利号ZL201810300609.9

12.  一种在单分子水平同时检测多种DNA糖基化酶的荧光化学传感器及其检测方法和应用，专利号ZL201711027692.9

13.  一种量子产率的测试方法，专利号ZL201310120635.0

14.  多组分生物标识物的检测方法，专利号ZL201010167849.X
学术成果